Device for extracting biological molecules from tissue...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C12M 1/34 (2006.01)

Patent

CA 2563613

The present invention provides a non-destructive macromolecules extraction (NDME) device for extracting high quantity of biological molecules (including, but not limited to, proteins, DNA, and/or RNA) from tissue specimens (including, but not limited to, fresh or fixed tissue sections, homogenized tissues, and cell cultures), while preserving the morphology and antigenicity of the tissue. The device contains a base, a slide cover, and a thermal control device. The tissue specimen is placed onto the base. The slide cover is mounted to the base to form a space where an extraction solution can be added. The device optionally contains a chamber cover over the slide cover. The chamber cover and the slide cover forms a reaction chamber where a steam is infused to maintain the humidity of the tissue. The extraction solution contains a detergent. The present invention also provides a method for extracting the same.

La présente invention fournit un appareil d'extraction des macromolécules (NDME) non-destructif pour réaliser l'extraction de grandes quantités de molécules biologiques (incluant, mais sans s'y limiter, protéines, ADN et/ou ARN) dans les échantillons de tissu (incluant, mais sans s'y limiter, coupes fraîches ou fixées, tissus homogénéisés et cultures cellulaires), tout en préservant la morphologie et l'antigénicité du tissu. L'appareil comporte une base, une protection des coupes et un système de régulation thermique. L'échantillon de tissu est placé sur la base. La protection des coupes est montée sur la base pour former un espace dans lequel une solution d'extraction peut être ajoutée. En option, l'appareil peut contenir une protection de la chambre placée par dessus la protection des coupes. La protection de la chambre et la protection des coupes forment une chambre de réaction où la vapeur est infusée pour maintenir l'humidité du tissu. La solution d'extraction contient un détergent. La présente invention fournit aussi un procédé pour le même type d'extraction.

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