Device for extraction of nucleic acids

Details Device for extraction of nucleic acids Device for extraction of nucleic acids

C - Chemistry, Metallurgy

12

N

C12N 15/00 (2006.01) B01F 11/00 (2006.01) B01F 13/00 (2006.01) B01L 3/00 (2006.01) C12Q 1/68 (2006.01) G01N 1/34 (2006.01) G01N 1/40 (2006.01) B01L 3/02 (2006.01) B01L 3/14 (2006.01)

Patent

CA 2390354

Nucleic acids are extracted rapidly, safely and directly from a sample without pipetting steps by using predispensed, interconnectable vessels. These vessels are used separately or interconnected according to the mictrotitre standard format. The sample is mixed with lysis buffer and the nucleic acids bound to a matrix in a closed system, comprising at least two interconnectable volumes. By forcing the sample and buffer mixture back and forth from one volume to another, passing a narrow passage, thorough mixing is ensured.

Des acides nucléiques sont extraits rapidement, sûrement et directement d'un échantillon sans opération de pipettage à l'aide de récipients interconnectables mis à disposition. Ces récipients sont utilisés séparément ou interconnectés conformément au format standard de microtitration. L'échantillon est mélangé à un tampon de lyse et les acides nucléiques sont liés à une matrice dans un système fermé comprenant au moins deux contenants interconnectables. On assure l'homogénéité du mélange en déplaçant en va-et-vient l'échantillon et le mélange tampon d'un contenant à un autre en les faisant passant par un passage étroit.

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