G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 27/447 (2006.01) G01N 30/92 (2006.01) H01J 49/04 (2006.01) H01J 49/06 (2006.01)
Patent
CA 2476493
A device for separating one or more components in a sample is disclosed, the device including: (a) a first planar member (1) having a channel (2) along which a sample may be loaded and component(s) thereof focussed isoelectrically; and (b) means for exposing the channel (2) along at least a portion of its length and thereby exposing the sample or component(s) therewithin. The means for exposing the channel (2) may include a cover plate (7). A method of analysing a molecule is also provided, the method including: (a) providing an elongate open channel (2); (b) introducing a plurality of molecules into the elongate open channel (2); (c) separating molecules along the elongate open channel (2) according to their isoelectric points; (d) accessing a molecule in the elongate open channel (2) and analysing it. A use of the device in combination with a mass spectrometer, preferably a MALDI-TOF unit, for analysis of a protein is also disclosed.
L'invention porte sur un dispositif permettant de s~parer un ou plusieurs des composants d'un ~chantillon, comprenant: (a) un premier ~l~ment plan (1) comportant un canal (2) le long duquel on peut charger l'~chantillon dont un ou des composants sont focalis~s iso~lectriquement, et (b) et des moyens d'exposition du canal (2) sur au moins une partie de sa longueur, et par-l~, de l'~chantillon ou d'un ou de plusieurs de ses composants, le moyen d'exposition du canal (2) pouvant consister en une plaque de couverture (7). L'invention porte ~galement sur un proc~d~ d'analyse de mol~cules consistant ~: (a) cr~er un canal (2) ouvert allong~; (b) introduire des mol~cules dans le canal (2); (c) r~partir et s~parer les mol~cules le long du canal (2) en fonction de leurs points iso~lectriques; (d) acc~der ~ une mol~cule pr~sente dans le canal (2); et (e) l'analyser. L'invention porte en outre sur l'utilisation du dispositif en association avec un spectromtre de masse, de pr~f~rence du type MALDI-TOF, pour l'analyse de prot~ines.
Genome Institute Of Singapore
Smart & Biggar
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