C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/02 (2006.01) C12M 3/00 (2006.01) G01N 33/50 (2006.01)
Patent
CA 2357846
A porous membrane is coated with a composition which includes a reconstituted and aggregated extracellular matrix derived from the Englebreth-Holm-Swarm mouse tumor, a polyol and a pH 7.8-8.2 buffer. The coated membrane is dried, affixed to an insert portion of an assembly and received in a well of a multiwell tissue culture plate. The invention includes a method to make the coated membrane.
Une membrane poreuse est revêtue d'une composition qui comprend une matrice extracellulaire reconstituée et agrégée dérivée d'une tumeur de souris Englebreth-Holm-Swarm, d'un polyol et d'une solution tampon de pH de 7,8 à 8,2. La membrane revêtue est séchée, fixée à une portion d'insert d'un ensemble et reçue dans un puits d'une plaque de culture à puits multiples. L'invention comprend une méthode de fabrication de la membrane revêtue.
Flaherty Paula
Ilsley Stephen R.
Kramer Martin L.
Mannuzza Frank J.
Becton Dickinson And Company
Gowling Lafleur Henderson Llp
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