B - Operations – Transporting – 01 – L
Patent
B - Operations, Transporting
01
L
B01L 3/00 (2006.01) B01D 61/18 (2006.01) B01D 61/28 (2006.01) B01D 63/02 (2006.01) B01J 19/00 (2006.01) B01L 3/02 (2006.01) B01L 7/00 (2006.01) C12Q 1/68 (2006.01) G01N 35/00 (2006.01)
Patent
CA 2369363
The present invention provides an automated device to facilitate rapid processing of sub-microliter samples using chemical or enzymatic reactions that involve liquid handling, one or more controlled temperature incubation steps (including temperature cycling), and a purification step based on molecular size discrimination. Exemplary applications include but are not limited to polymerase chain reaction (PCR), DNA sequencing applications, oligonucleotide ligation, ligase chain reaction (LCR), single nucleotide extension, exonuclease treatment, and oligonucleotide hybridization assays.
L'invention concerne un dispositif automatique, permettant de traiter rapidement des échantillons de la taille du sous-microlitre, par réactions chimiques ou enzymatiques qui impliquent une manipulation de liquide, une ou plusieurs étapes d'incubation à température régulée (notamment le thermocyclage), et une étape de purification basée sur la discrimination de taille moléculaire. On compte, notamment, parmi les applications exemplaires, la réaction en chaîne de la polymérase (PCR), des applications de séquençage d'ADN, la ligation d'oligonucléotides, la ligation répétitive d'oligonucléotides (LCR), l'extension unique de nucléotides, le traitement par un exonucléase, et des dosages d'hybridation d'oligonucléotides.
Cahill Patrick
Engelstein Marcy
Smith Douglas
Thomann Ulrich
Genome Therapeutics Corporation
Gowling Lafleur Henderson Llp
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