Device for the study of organotypic cultures and its uses in...

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/34 (2006.01) G01N 27/00 (2006.01) G01N 33/483 (2006.01) G01N 33/487 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2191974

The present invention relates to the domain of electrophysiology and biochemistry and to the measuring devices in these domains. A more particular subject of the invention is a new device which enables tissular explants to be kept alive and the electrophysiological and biochemical activity of the tissue studied to be continuously collected and analyzed. This device is formed by two half-cards forming the upper part and the lower part respectively of the interface which are fitted together to form a card intended to be inserted in an electronic module specially designed for this purpose. This device is used for measuring the electrophysiological activity of excitable cells using a biocompatible multielectrode array and one or more microdialysis probes for biochemical or radioimmunoassays.

La présente invention se rapporte aux domaines de l'électrophysiologie et de la biochimie, ainsi qu'aux dispositifs de mesure utilisés dans ces domaines. L'invention se rapporte tout particulièrement à un nouveau dispositif qui permet de conserver des explants tissulaires vivants et de mesurer et d'analyser en continu l'activité électrophysiologique et biochimique du tissu à l'étude. Ce dispositif est constitué de deux demi-cartes formant respectivement la partie supérieure et la partie inférieure de l'interface et qui sont assemblées pour former une carte destinée à être insérée dans un module électronique conçu spécialement pour cette étude. Ce dispositif est utilisé pour mesurer l'activité électrophysiologique de cellules excitables à l'aide d'un réseau multiélectrode biocompatible et d'au moins une sonde de microdialyse en vue de réaliser des dosages biochimiques ou radio-immunologiques.

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