Device for use in the isolation of a biological material...

Details Device for use in the isolation of a biological material... Device for use in the isolation of a biological material...

B - Operations, Transporting

01

D

B01D 15/00 (2006.01) C07H 1/08 (2006.01) C12N 15/10 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2198757

The present invention relates to the isolation of a biological material, for example nucleic acid, from a basic material containing said biological material. A device is provided characterized by a container (1) for holding a mixture of the basic material, a chaotropic substance and a solid phase which binds the biological material; means (10) for separating the solid phase with the biological material from the fluid bound thereto; and means for connecting the contaianer (1) to an inlet (6) and outlet (9) for washing fluid for washing the biological material bound to the solid phase, to an inlet (6) for an eluent fluid, and to an eluate reservoir (17) for collection of the eluant fluid with the dissolved biological material.

L'invention concerne l'isolement d'une substance biologique telle que l'acide nucléique dans une matière de base contenant ladite substance. Pour ce faire, on prévoit un dispositif constitué d'un récipient (1) conçu pour conserver un mélange de la matière de base, une substance chaotrope et une phase solide se liant à la substance biologique; d'un moyen de séparation (10) de la phase solide et de la substance biologique du fluide auquel elles sont liées; et d'un moyen pour relier le récipient (1) à une entrée (6) et une sortie (9) pour le fluide de lavage destiné à laver la substance biologique liée à la phase solide, et à un réservoir d'éluat (17) conçu pour recueillir l'éluat fluide ainsi que la substance biologique dissoute.

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