Devices and methods for coupling mass spectrometry devices...

Details Devices and methods for coupling mass spectrometry devices... Devices and methods for coupling mass spectrometry devices...

G - Physics

01

N

G01N 1/34 (2006.01) G01N 30/20 (2006.01) G01N 35/00 (2006.01) H01J 49/04 (2006.01) G01N 30/96 (2006.01)

Patent

CA 2703991

One embodiment of the invention provides a method of preparing an eluted sample containing salts or buffers from a liquid chromatography device for analysis by a mass spectrometry device. The method includes: continuously providing a non-polar solvent to the mass spectrometry device; receiving the eluted sample from the liquid chromatography device; flowing the eluted sample over a solid phase extraction column; flowing the non-polar solvent over the solid phase extraction column; and presenting non-polar solvent and the eluted sample to the mass spectrometry device.

Un mode de réalisation de la présente invention concerne un procédé de préparation d'un échantillon élué contenant des sels ou des tampons en provenance d'un dispositif de chromatographie liquide en vue de son analyse au moyen d'un dispositif de spectrométrie de masse. Ce procédé comprend les étapes consistant à alimenter en continu le dispositif de spectrométrie de masse en un solvant non polaire; à recevoir l'échantillon élué en provenance du dispositif de chromatographie liquide; à faire circuler l'échantillon élué dans une colonne d'extraction en phase solide; à faire circuler le solvant non polaire dans la colonne d'extraction en phase solide; et à soumettre le solvant non polaire et l'échantillon élué au dispositif de spectrométrie de masse.

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