Devices and methods for detecting cells and other analytes

G - Physics – 01 – N

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Details

G01N 33/53 (2006.01) B01L 3/00 (2006.01) C12M 1/00 (2006.01) C12M 1/34 (2006.01) C12Q 1/68 (2006.01) G01N 15/06 (2006.01)

Patent

CA 2646293

The invention features methods, devices, and kits for the isolation of analytes (e.g., a cell). A sample containing a desired analyte is introduced into a microfluidic device containing moieties that bind the desired analyte. A shear stress is applied that is great enough to prevent binding of undesired analytes and low enough to allow binding of the analyte of interest. Once bound, the desired analytes can be analyzed (e.g., counted). The invention also features methods for determining a shear stress for isolating a desired analyte.

L'invention concerne des procédés, des dispositifs et des kits pour l'isolation d'analytes (par exemple une cellule). Un échantillon contenant un analyte souhaité est introduit dans un dispositif microfluidique contenant des fragments qui se lient à l'analyte souhaité. On applique une contrainte de cisaillement qui est suffisamment forte pour empêcher la liaison d'analytes non souhaités et suffisamment faible pour permettre la liaison de l'analyte auquel on s'intéresse. Une fois liés, les analytes souhaités peuvent être analysés (par exemple comptés). L'invention concerne aussi des procédés pour déterminer une contrainte de cisaillement pour isoler un analyte souhaité.

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