Devices and methods for production of cell aggregates

B - Operations – Transporting – 01 – L

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Details

B01L 3/00 (2006.01) C12M 1/00 (2006.01) C12M 1/26 (2006.01) C12M 1/32 (2006.01) C12M 3/00 (2006.01) C12N 5/00 (2006.01) C12Q 1/24 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2679011

The present application provides methods and devices for the production and recovery of cell aggregates. In one embodiment, the device is a microwell device with a high density of microwells. The application also provides a device for extracting cell aggregates such as stem cells or embryoid bodies from well plates. Such cell aggregates are used for the differentiation of pluripotent stem cells such as embryonic stem cells, in the fields of developmental biology and regenerative medicine / tissue engineering.

La présente invention concerne des procédés et des dispositifs de fabrication et de récupération d'agrégats cellulaires. Dans un mode de réalisation, le dispositif est un dispositif à micropuits présentant une densité élevée de micropuits. L'invention concerne également un dispositif permettant d'extraire des plaques de puits les agrégats cellulaires tels que les cellules souches ou les corps embryoïdes. De tels agrégats cellulaires sont utilisés pour la différenciation de cellules souches pluripotentes telles que les cellules souches embryonnaires, dans les domaines de la biologie développementale et de la médecine régénérative/du génie génétique tissulaire.

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