Diacetyldichlorofluorescin with a source of hydrogen...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

167/43, 150/15.1

C12Q 1/26 (2006.01) C12Q 1/28 (2006.01) G01N 33/535 (2006.01)

Patent

CA 1144859

Abstract An assay for substances demonstrating peroxidative activity is based on the peroxidase catalyzed oxidation of dichlorofluoresein to dichlorofluorescein by peroxide. The dichloro- fluoresein is formed in situ from diacetyldichloro- fluoresein either by the direct addition of peroxide or by the inclusion of a peroxide generating system in the reaction mixture.

346350

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