Diacylglycerol acyl transferase proteins

Details Diacylglycerol acyl transferase proteins Diacylglycerol acyl transferase proteins

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) A61K 39/395 (2006.01) A61P 3/00 (2006.01) A61P 9/00 (2006.01) A61P 35/00 (2006.01) C07K 16/40 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01) G01N 33/566 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2331329

The invention provides diacylglycerol acyl transferase (DAGAT) proteins, wherein said proteins are active in the formation of triacylglycerol from fatty acyl and diacylglycerol substrates. In one aspect, <i>Mortierella ramanniana</i> DAGAT proteins have been isolated and have molecular weights of between approximately 36 and 37kDa as measured by SDS-PAGE. The invention also provides novel DAGAT polynucleotide and polypeptide sequences and to methods of producing such polypeptides using recombinant techniques. In addition, methods are provided for using such sequences to alter triacylglycerol levels in plants and to treat diseases associated with altered DAGAT activity or expression.

Protéines de diacylglycérol acyl transférase (DAGAT) qui sont actives dans la formation de triacylglycérol à partir de substrats gras acyle et diacylglycérol. Selon un aspect de la présente invention, des protéines DAGAT de <i>Mortierella ramanniana</i> ont été isolées et possèdent des poids moléculaires se situant approximativement entre 36 et 37 kDa tels que mesurés par l'électrophorèse sur gel SDS. La présente invention concerne également de nouvelles séquences polynucléotidiques et polypeptidiques de DAGAT et des procédés de production desdits polypeptides à l'aide de techniques de recombinaison. En outre, des procédés d'utilisation desdites séquences pour modifier les taux de triacylglycérol dans des végétaux et de traitement de maladies associées à une modification de l'activité ou de l'expression de DAGAT sont également décrits.

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