Diacylglycerol acyltransferase 2 genes and proteins encoded...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/10 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A23D 9/00 (2006.01) C12N 15/54 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2709067

The present disclosure relates to the isolation, purification, and characterization of a diacylglycerol acyltransferase 2 (DGAT2), and genes encoding DGAT2, from algae. DGAT2 can incorporate very long chain polyunsaturated fatty acids in to triacylglycerol more efficiently than DGAT1. The disclosure concerns methods of regulating seed oil content, fatty acid synthesis and fatty acid composition using the DGAT2 gene and to tissues and plants transformed with the gene. The disclosure also relates to transgenic plants, plant tissues and plant seeds having a genome containing an introduced DNA sequence of the disclosure, and a method of producing such plants and plant seeds.

La présente invention concerne l'isolement, la purification et la caractérisation de diacylglycérol acyltransférase 2 (DGAT2), et de gènes codant DGAT2, à partir d'algues. DGAT2 peut incorporer des acides gras polyinsaturés à très longue chaîne dans un triacylglycérol plus efficacement que DGATl. L'invention concerne des procédés de régulation de la teneur en huile de graines, de la synthèse d'acides gras et de la composition en acides gras au moyen du gène DGAT2 et des tissus et des plantes transformés par le gène. L'invention concerne également des plantes, des tissus végétaux et des graines de plantes transgéniques dont le génome contient une séquence d'ADN introduite de l'invention, et un procédé de production de ces plantes et graines de plantes.

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