Diacylglycerol acyltransferase assay

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/61 (2006.01)

Patent

CA 2586477

The present invention generally provides a method of measuring the biological activity of diacylglycerol acyltransferase (DGAT). Specifically, the present invention provides a method for rapid, mass screening of compounds which are able to modulate the biological activity of DGAT. More specifically, the present invention provides an assay system for measuring DGAT activity based on the use of particular micelles with the FlashPlate~ technology.

La présente invention porte, de manière générale, sur une méthode permettant de mesurer l'activité biologique de diacylglycérol acyltransférase (DGAT). De manière spécifique, invention porte sur une méthode de recherche systématique et rapide de composés capables de moduler l'activité biologique de DGAT. Plus spécifiquement, invention porte sur un système de deux flashes permettant de mesurer l'activité de DGAT en utilisant des micelles particulières selon la technologie FlashPlate~.

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