Diagnosis and treatment of myocardial failure

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) A01N 43/04 (2006.01) C07H 21/04 (2006.01) C07K 14/47 (2006.01) C12N 15/00 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2278465

The invention provides methods and kits for diagnosing myocardial failure in a human. One method comprises obtaining a sample of myocardial tissue from a ventricle of the heart of the human; quantitating the expression of alpha-myosin heavy chain (alpha-MHC), beta-myosin heavy chain (beta-MHC), or both in the sample; and determining by statistical analysis if the expression of alpha-MHC or beta--MHC, or both in the sample is significantly different than their expression in human ventricular myocardial tissue. The invention also comprises a method of treating myocardial failure in a human.

L'invention concerne un procédé permettant de diagnostiquer une défaillance myocardique chez un être humain. Le procédé comporte les étapes consistant à obtenir un échantillon de tissu myocardique provenant d'un ventricule de coeur humain; quantifier l'expression de chaînes lourdes d'alpha-myosine (alpha-MHC), de chaînes lourdes de bêta-myosine (bêta-MHC), ou des deux à la fois dans l'échantillon; et déterminer par analyse statistique si l'expression d'alpha-MHC ou de bêta-MHC, ou des deux ensemble dans l'échantillon est significativement différente de leur expression dans un tissu myocardique ventriculaire humain normal.

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