Diagnosis of helicobacter pylori with surface plasmon resonance

Details Diagnosis of helicobacter pylori with surface plasmon resonance Diagnosis of helicobacter pylori with surface plasmon resonance

G - Physics

01

N

G01N 33/569 (2006.01) C07K 14/205 (2006.01)

Patent

CA 2472230

The present invention relates to novel methods for the diagnosis of Helicobacter pylori infection. Specifically, the present invention relates to novel non-invasive methods for the detection of the presence or absence of a Helicobacter pylori antigen or a metabolite produced by the bacterium in a biological sample with a biosensor-based measurement. The present invention also related to the use of a biosensor containing specific antibodies against H. pylori or antigen-binding fragments thereof immobilized thereto together with biomolecule-repellent polymers preventing the non-specific binding. The invention also relates to test kits useful in the methods.

L'invention concerne de nouvelles méthodes permettant de poser le diagnostic d'une infection à Helicobacter pylori. D'une manière plus spécifique, l'invention concerne de nouvelles méthodes non invasives permettant de détecter, au moyen d'une mesure effectuée avec un biodétecteur, la présence ou l'absence, dans un échantillon biologique, d'un antigène Helicobacter pylori ou d'un métabolite produit par la bactérie. L'invention concerne également l'utilisation d'un biodétecteur contenant des anticorps spécifiques dirigés contre H. pylori ou des fragments Fab de H. pylori immobilisés conjointement avec des polymères à répulsion biomoléculaire qui bloquent la liaison non spécifique. L'invention concerne également des trousses d'essai utiles dans lesdites méthodes.

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