Diagnosis of whipple disease

Details Diagnosis of whipple disease Diagnosis of whipple disease

C - Chemistry, Metallurgy

12

N

C12N 1/00 (2006.01) C07K 16/12 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2367989

The invention relates to a method for in vitro seriological diagnosis of Whipple disease, whereby the bacteria responsible for said disease is isolated and established in a culture and brought into contact with the serum of biological fluid of a patient. The invention also relates to useful oligonucleotides with a probe and a primer for amplification, sequencing and detection of the gene rpoB of bacteria Tropheryma whippelii.

L'invention concerne une méthode pour le diagnostic sérologique in vitro de la maladie de Whipple, selon laquelle on met en contact la bactérie responsable de ladite maladie, isolée et établie en culture, avec le sérum ou le fluide biologique d'un patient. L'invention concerne également un dispositif pour la mise en oeuvre de cette méthode, ainsi qu'une trousse pour la détection in vitro de la bactérie responsable de la maladie de Whipple. L'invention concerne également des oligonucléotides utiles avec sonde et amorce pour l'amplification, le séquençage et la détection du gène rpoB de la bactérie Tropheryma whippelii.

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