C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/04 (2006.01)
Patent
CA 2395938
The invention provides a method for analysing for bacteria in a liquid sample comprising the following steps: a) contacting said sample with a substrate capable of retaining bacteria or bacterial fragments; b) staining any bacteria retained on said substrate with at least one solution comprising a first dye, said staining being conducted at a pH at which the dye has an overall positive charge and the bacteria has an overall negative charge; and c) inspecting the substrate for the presence of a stain whereby to indicate the presence of bacteria in said sample; with the proviso that where said liquid sample is other than a milk sample and substrate is a filter then said filter comprises a polysulfone or a derivative of a polysulfone.
L'invention concerne une méthode permettant d'analyser des bactéries dans un échantillon liquide, consistant à a) mette ledit échantillon en contact avec un substrat capable de retenir des bactéries ou des fragments de celles-ci; b) à colorer toute bactérie retenue sur ledit substrat à l'aide d'au moins une solution comprenant un premier colorant, la coloration étant effectuée à un pH auquel le colorant possède une charge globale positive, et les bactéries une charge globale négative; et c) à rechercher la présence d'une coloration sur le substrat, ce qui indique la présence de bactéries dans l'échantillon; à condition que ledit échantillon liquide soit autre qu'un échantillon de lait, et que le substrat soit un filtre renfermant un polysulfone ou un dérivé de polysulfone.
Gogstad Geir Olav
Larsen Hans Jorgen Soiland
Sorum Rune Henning
Yazdankhah Siamak Pour
Kirby Eades Gale Baker
Procaryo As
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