Diagnostic applications of double d-loop formation

Details Diagnostic applications of double d-loop formation Diagnostic applications of double d-loop formation

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2116215

The present invention describes the formation of RecA protein catalyzed double-stranded probe:duplex linear target DNA complexes that are stable to deproteinization. The uses of this stable probe:target complex in diagnostic/DNA detection systems in in vitro and in situ DNA hybridization reactions is discussed. The probe:target complexes are also useful for diagnostic application in RecA protein facilitated DNA amplification reactions.

L'invention décrit la formation de complexes composés d'une sonde à double brin catalysée par la protéine RecA et de l'ADN cible linéaire double hélice et qui ne sont pas altérés par la déprotéinisation. Les utilisations de ce complexe stable sonde:cible dans les systèmes de détection d'ADN et de diagnostic dans les réactions d'hybridation d'ADN in vitro et in situ sont discutées. Ces complexes sont également utiles pour l'application diagnostique dans les réactions d'amplification de l'ADN facilitées par la protéine RecA.

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