Diagnostic assay for cancer susceptibility

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12Q 1/34 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2308766

The lifetime probability of a person developing cancer can now be determined based on an allelic variation found in the 3'UTR of the prohibitin gene. The probability is dependent on the sequence of the 3'UTR at position 729, i.e., whether there is a thymine (T) or a cytosine (C) or both at this position. Determining the sequence at the position 729 can be done by any number of standard techniques. Preferably, the sequence is determined by amplifying this region by PCR and subjecting it to an RFLP analysis.

La probabilité pour une personne de développer un cancer au cours de sa vie peut maintenant être déterminée sur la base de la variation allélique trouvée dans la séquence non traduite 3' (3'UTR) du gène de prohibitine. La probabilité dépend de la séquence 3'UTR à la position 729, en d'autres termes de la présence ou non d'une thymine (T) et/ou d'une cytosine (C) à cette position. La détermination de la séquence à la position 729 peut être opérée à l'aide de n'importe quelle technique standard. De préférence, on détermine la séquence par amplification en chaîne par polymérase (PCR) de cette région, puis on soumet ladite région à l'analyse des fragments de restriction polymorphes (RFLP).

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