Diagnostic assay for measuring a cell mediated immune response

Details Diagnostic assay for measuring a cell mediated immune response Diagnostic assay for measuring a cell mediated immune response

G - Physics

01

N

G01N 33/53 (2006.01) C12Q 1/68 (2006.01) G01N 33/567 (2006.01)

Patent

CA 2504829

The present invention relates generally to a diagnostic assay and, more particularly, an assay for measuring cell-mediated immune reactivity. Even more particularly, the present invention provides an assay and a kit for measuring a cell-mediated response to an antigen using whole blood or other suitable biological sample. The assay may be conducted using ligands to immune effector molecules or at the nucleic acid level, screening for expression of genes encoding the immune effector molecules. The assay is useful in therapeutic and diagnostic protocols for human, livestock and veterinary and wild life applications.

La présente invention concerne de façon générale une méthode diagnostique et, plus particulièrement, un dosage permettant de mesurer la réactivité immunitaire à médiation cellulaire. Ladite invention concerne encore plus particulièrement un dosage et une trousse permettant de mesurer une réponse à médiation cellulaire à un antigène au moyen de sang total ou d'un autre échantillon biologique approprié. Ce dosage peut être effectué au moyen de ligands liés à des molécules effectrices de la réponse immunitaire ou au niveau de l'acide nucléique par l'identification de l'expression de gènes codant pour ces molécules effectrices de la réponse immunitaire. Ce dosage s'utilise dans des protocoles diagnostiques et thérapeutiques en médecine humaine et vétérinaire, chez les animaux d'élevage ainsi que dans la nature.

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