Diagnostic detection device

G - Physics – 01 – N

Patent

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Details

G01N 33/53 (2006.01) G01N 33/558 (2006.01) G01N 33/74 (2006.01) G01N 33/76 (2006.01)

Patent

CA 2679334

The invention comprises a device for detecting an analyte in a liquid sample deposited on a first portion of the device for transport to a second portion of the device that is in fluid contact with the first portion. In specific embodiments, the device comprises a labeled conjugate comprising a binding member reactive with a first epitope of the analyte and a label comprising a gold colloid, preferably having a mean particle size of 50 nm to 100 nm. In further embodiments, the device comprises a capture component comprising polymerized streptavidin. The diagnostic device is particularly useful in the preparation of pregnancy test kits.

La présente invention concerne un dispositif destiné à détecter un analyste dans un échantillon liquide déposé sur une première partie de ce dispositif en vue d'un transport vers une seconde partie de ce dispositif en contact fluidique avec la première partie. Dans des modes de réalisation spécifiques de l'invention, le dispositif comprend un conjugué marqué comprenant un élément de liaison qui réagit avec un premier épitope de l'analyse et un marqueur comprenant de l'or colloïdal, de préférence possédant une taille de particule moyenne comprise entre 50 nm et 100 nm. Dans d'autres modes de réalisation de l'invention, le dispositif comprend un élément de capture comprenant une streptavidine polymérisée. Ce dispositif de diagnostic convient particulièrement pour la préparation de trousses de test de grossesse.

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