Diagnostic device for rapid determination of buprenorphine

Details Diagnostic device for rapid determination of buprenorphine Diagnostic device for rapid determination of buprenorphine

G - Physics

01

N

G01N 33/94 (2006.01) B01L 3/00 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2502402

This invention relates to a method of detecting buprenorphine in biological fluids (especially urine), and to a device and kit which use chromatographically mobile reagents labelled with gold clusters. The method involves contacting urine sequentially with anti- buprenorphine antibodies labelled with gold clusters and with buprenorphine immobilised on a porous support, in order to detect the presence of buprenorphine in the sample by means of a competitive reaction. The device according to the invention consists of a rack which can be filled with a number of strips; the kit consists of a box with separate sections for the strips and a separate compartment which holds the rack.

L'invention concerne un procédé de détection de buprénophine dans des fluides biologiques (en particulier l'urine), et un dispositif et un kit qui utilisent des réactifs mobiles de manière chromatographique marqués au moyen d'agrégats d'or. Ledit procédé implique la mise en contact de l'urine de manière séquentielle avec des anticorps anti-buprénophine marqués d'agrégats d'or, de façon à détecter la présence de buprénophine dans l'échantillon au moyen d'une réaction compétitive. Le dispositif de l'invention est constitué d'un support qui peut être rempli par de nombreuses bandes. Le kit est constitué d'une boîte à sections séparées pour les bandes et un compartiment séparé qui maintient le support.

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