Diagnostic immunochemical test material and procedure

Details Diagnostic immunochemical test material and procedure Diagnostic immunochemical test material and procedure

G - Physics

01

N

167/45

G01N 33/554 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1104927

DIAGNOSTIC IMMUNOCHEMICAL TEST MATERIALS AND PROCEDURE ABSTRACT OF THE DISCLOSURE Materials and procedure are disclosed for conducting a rapid diagnostic test for the detection of an immunochem- ically reactive substance. The test is suitable for detection of a specific antigen or antibody in serum or other bodily fluid. A substance capable of immunochemically reacting with the substance to be detected is immobilized on an insoluble carrier. The immobilized substance is used to harvest the substance to be detected from the bodily fluid. The presence of the harvested substance is detected by the use of an antibody-enzyme conjugate capable of immunochemically binding the harvested substance. The immunochemically bound enzyme activity provides a direct measure of the amount of the harvested substance. Proper choice of carrier for the immobilized substance, method of immobilization, reagents and method of conducting the reaction sequence results in a rapid, sensitive, convenient and inexpensive technique, especially useful for detecting antibodies to Toxoplasma gondii and antigens of viruses, such as Hepatitis B.

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