C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/8.5
C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2002076
-0- DIAGNOSTIC KIT AND METHOD USING A SOLID PHASE CAPTURE MEANS FOR DETECTING NUCLEIC ACIDS Abstract of the Disclosure A method for the detection of a specific nucleic acid sequence of a predetermined nucleic acid in a specimen includes the use of oligonucleotide primers complementary to the sequence, a labeled probe and known amplification techniques to form primer extension products of the primers. At least one of the primers is labeled with a specific binding moiety which, at some point in the method, is complexed with a receptor thereto. The receptor is bound to a solid support material, such as polymeric particles. The complexation can occur either prior or subsequent to the formation of primer extension products. Thus, the detectable complex is captured on the solid support material for convenient detection. A diagnostic test kit comprises the primers, an immobilized receptor, a polymerization agent and the deoxyribonucleotide triphosphates needed for carrying out the amplification and detection of the specific nucleic acid sequence.
Burdick Brent A.
Chang Chu-An
Fyles Janet L.
Levenson Corey H.
Oakes Fred T.
Burdick Brent A.
Cetus Corporation
Chang Chu-An
Eastman Kodak Company
Fyles Janet L.
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