Diagnostic kit for simultaneously detecting multiple...

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G01N 33/544 (2006.01) G01N 33/571 (2006.01) G01N 33/576 (2006.01)

Patent

CA 2422942

The present invention relates to a kit for the simultaneous diagnosis of a plurality of infectious diseases and a method for preparation of it. The kit comprises a colloidal gold-labeled inmmunofiltration device, a buffer solution and a mixture of colloidal gold labels, wherein the HBsAg monoclonal antibody, the HCV antigen, the syphilis antigen the HIV antigen and the sheep anti-mouse IgM antibody label are immobilized in dots in the nitrocellulose membrane of the colloidal gold-labeled immunofiltration device. The present invention also relates to a method for preparation of said kit. The kit of the present invention carries out the simultaneous diagnosis of the infectious diseases comprising B hepatitis, C hepatitis, syphilis and AZT, bases on the principle of colloidal gold immunofiltration method, which is operated simply and rapidly and accurately, and is suitable for the detection of a plurality of diseases in various blood samples, in particularly is suitable for the detection of large- scale blood samples to provide a new approach for the detection of infectious diseases.

La présente invention concerne un kit de diagnostic simultané d'une pluralité de maladies infectieuses et une technique de préparation de celui-ci. Ce kit comprend un dispositif d'immunofiltration à marqueur or colloïdal, une solution tampon et un mélange de marqueurs or colloïdal. L'anticorps monoclonal HbsAg, l'antigène du VHC, l'antigène de la syphilis, l'antigène du VIH, et le marqueur d'anticorps IgM mouton anti-souris sont immobilisés dans des points de la membrane de nitrocellulose du dispositif d'immunofiltration à marqueur or. Cette invention concerne aussi une technique de préparation de ce kit. Le kit de cette invention effectue simultanément un diagnostic de maladies infectieuses comprenant l'hépatite B, l'hépatite C, la syphilis et l'AZT. Ces diagnostics sont fondés sur la technique d'immunofiltration à marqueur or colloïdale qui est réalisée simplement, rapidement et précisément et qui convient pour détecter une pluralité de maladies dans divers prélèvement sanguins, et en particulier pour la détection de prélèvements sanguins à grande échelle de façon à offrir une nouvelle méthode de détection de maladies infectieuses.

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