G - Physics – 01 – N
Patent
G - Physics
01
N
150/3, 167/37, 1
G01N 33/53 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2013317
-0- DIAGNOSTIC KIT, PRIMER COMPOSITION AND THEIR USE FOR REPLICATION OR DETECTION OF NUCLEIC ACIDS Abstract of the Disclosure Amplification, replication or detection of a predetermined target nucleic acid can be carried out using a unique primer composition. This composition comprises an aqueous mixture of a first oligonucleotide primer which is substantially complementary to a first specific nucleic acid sequence of the target, but which is suspected of having one or more mismatches with the target at or near its 3' end. Also included in the composition is one or more additional primers which are complementary to a nucleic acid sequence of the target. This sequence is either: (i) inclusive of only a portion of the first specific nucleic acid sequence, (ii) immediately adjacent to the first specific nucleic acid sequence, or (iii) removed from the first specific nucleic acid sequence by one or more bases, but which additional primer is capable of forming a primer extension product complementary to the first specific nucleic acid sequence. These composition components can be supplied as part of a diagnostic test kit which can include other reagents if desired.
Eastman Kodak Company
Gowling Lafleur Henderson Llp
Oakes Fred T.
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