Diagnostic kits and methods for making granulocyte cell counts

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/28 (2006.01) C12M 1/00 (2006.01) C12Q 1/04 (2006.01) G01N 33/49 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2113101

2113101 9301306 PCTABS00019 Methods for detecting granulocytes and for providing an at least semiquantitative indication of granulocyte level and kits for implementing those methods. A blood sample is drawn, and components that might interfere with the assay are removed and/or neutralized. Granulocytes present in the sample are then disrupted to release intracellular myeloperoxidase, and the sample is contacted with a peroxide and a chromogenic donor dye. The myeloperoxidase enzyme catalyzes hydrogen peroxide-involved reactions which result in the dye changing color if, and to the extent that, granulocytes are present in the blood sample.

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