Diagnostic method for proteinaceous binding pairs,...

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G01N 33/566 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2629451

Method of measuring the quantity of a first proteinaceous specific binding partner (sbp) in a biological sample comprising detecting the binding of the first proteinaceous sbp with a labeled second proteinaceous sbp, wherein neither the first or second sbp is an antibody or fragment thereof, which is preferably a method of determining the amount of sFlt-1, particularly free sFlt-1, and the amount of PlGF, particularly free PlGF, in a sample, which is preferably used in a method of predicting risk of preeclampsia comprising comparing free PlGF to free sFlt-1.

L'invention a pour objet un procédé de mesure quantitative d'un premier partenaire de liaison spécifique protéique (sbp) dans un échantillon biologique comprenant la détection de la liaison du premier sbp protéique avec un second sbp protéique marqué, ni le premier, ni le second sbp n'étant un anticorps ou un fragment d'anticorps, de préférence un procédé de détermination de la quantité de sFlt-1, notamment de sFlt-1 libre, et de la quantité de PlGF, notamment de PlGF libres, dans un échantillon, utilisé de préférence dans le cadre d'un procédé de prévision des risques de pré-éclampsie comprenant la comparaison des PlGF libres et de sFlt-1 libre.

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