G - Physics – 01 – N
Patent
G - Physics
01
N
358/5.04
G01N 23/00 (2006.01)
Patent
CA 1093221
Abstract A diagnostic reagent holder specifically adapted for the detection of an unknown quantity of a biologically derived sample suitably by radioim- munoassay, flurometric detection or by spectrophotometry. The holder com- prises an elongate shaft with a support surface adjacent one end of the shaft Diagnostic reagent is covalently attached to a self-supporting film such as a disc and firmly secured to the support. The disc may be retained in a groove in the support portion or may be adhered against the support surface. A pro- jection or recess may be provided on the support to assist registry thereof with a cooperating portion of a viewing housing. A pivot extension project- ing down from the holder forms a pivot point facilitating stirring. The dia- gnostic reagent, e.g., antibody, is stirred during incubation in contact with the sample to be detected, e.g., antigen, in solution. In a "sandwich" tech- nique labelled substance (e.g., fluorochrome-labelled antibody) is reacted with the sample on the film followed by thorough washing. Then, the holder is slid into the novel viewing housing which precisely positions the film at a window for viewing as with a fluorometer. To avoid loss of labelled sub- stance during handling, and especially during positioning in the viewing housing, one or more protective protrusions are provided around the periphery of the support surface.
350307
Deindoerfer Fred H.
Jang Roland
Rudow H. Theodore
International Diagnostic Technology Inc.
Smart & Biggar
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