Diagnostic reagent, kit and method employing polynucleotide...

Details Diagnostic reagent, kit and method employing polynucleotide... Diagnostic reagent, kit and method employing polynucleotide...

C - Chemistry, Metallurgy

12

Q

195/1.12, 150/8.

C12Q 1/68 (2006.01) C12Q 1/66 (2006.01)

Patent

CA 1249534

ABSTRACT A reagent complex is disclosed containing (1) a probe polynucleotide having a target binding region complementary to a target nucleotide sequence and (2) a signal strand polynucleotide bound by base pairing to a portion of the target binding region. The signal strand is displaced from the reagent complex by the target nucleotide sequence and separated from intact reagent complexes. A digestable ribonucleotide segment of the displaced signal strand, such as a 3' terminal segment, is digested to ribonucleotide phosphates, which are phosphorylated to ATP. The ribonucleotide phosphates or ATP are detected.

507929

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