G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/68 (2006.01) G01N 33/574 (2006.01)
Patent
CA 2123856
ABSTRACT OF THE DISCLOSURE A new diagnostic test and kit for diseases or disorders in the digestive system. This study describes method for extraction and quantification of calprotectin (L1 protein) in feces by enzyme immunoassay. This protein is a prominent antimicrobial component of neutrophils, monocytes, macrophages and squamous epithelia. Calprotectin was stable in feces during storage for seven days at room temperature. Fecal calprotectin quantitated in a five gram sample taken from a 24 hours feces collection gave a reliable estimate of calprotectin found in the pooled collection. The assay had a within assay precision (=CV) of 1,9 % and a between assay precision of 14,8 %. The following mean fecal calprotectin levels were found: Healthy subjects (n=33) 3095 µg/l, hospital controls (n=40): 14637 µg/l, patients with inflammatory bowel disease (Crohn's disease and ulcerative colitis), (n=28) 40850 µg/l. The differences between the means are highly significant. All patients with IBD and ten out of eleven patients with gastrointestinal carcinomas had calprotectin level above the suggested reference limit of 9000 µg/l. Determination of fecal calprotectin is an important routine parameter for monitoring IBD and gastrointestinal cancer.
Dale Inge
Fagerhol Magne K.
Rõseth Arne G.
Bereskin & Parr
Dale Inge
Fagerhol Magne K.
Rõseth Arne G.
LandOfFree
Diagnostic test and kit for disease or disorders in the... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Diagnostic test and kit for disease or disorders in the..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Diagnostic test and kit for disease or disorders in the... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1751213