Diagnostic test for mutations in codons 12-13 of human k-ras

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2741592

The invention is directed to compositions, methods and kits for diagnosing cancers and tumors correlated with mutations in codons 12 and 13 of human K-RAS using primers that amplify target sequences. The amplified target sequences are then analyzed by any number of mass spectrometric techniques, which data are queried against a database of base composition signatures of K-RAS mutations in codons 12 and 13.

L'invention porte sur des compositions, des procédés et des trousses pour diagnostiquer des cancers et des tumeurs corrélées à des mutations dans les codons 12 et 13 de K-RAS humain à l'aide d'amorces qui amplifient des séquences cibles. Les séquences cibles amplifiées sont ensuite analysées par n'importe quel nombre de techniques de spectrométrie de masse, lesquelles données sont interrogées en fonction d'une base de données de signatures de compositions de base de mutations de K-RAS dans les codons 12 et 13.

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