C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/37 (2006.01) G01N 1/28 (2006.01) G01N 33/53 (2006.01) C12P 21/06 (2006.01)
Patent
CA 2672972
The invention provides methods of preparing a test sample for use in an assay for detecting an analyte bound by an intracellular ligand. The methods typically involve contacting the test sample with an assay reagent comprising: a lysis reagent; and a protease that has proteolytic activity for said intracellular ligand; to form a mixture compatible for use in an immunoassay without subsequent extraction steps. Other aspects of the invention include related immunoassays and test kits.
Procédé de préparation d'un échantillon d'essai pour une analyse visant à détecter une substance liée à un ligand intracellulaire. De façon générale, le procédé consiste à mettre l'échantillon d'essai en contact avec un réactif comprenant: un réactif liquide; et une protéase à activité protéolytique pour ledit ligand intracellulaire, le but étant de former un mélange compatible pour utilisation dans une analyse immunologique sans opération d'extraction ultérieures. Autres aspects de l'invention: analyses immunologiques et des trousses d'essai connexes.
Ali Salman
Grenier Frank C.
Syed Hina N.
Workman Ryan F.
Abbott Laboratories
Gowling Lafleur Henderson Llp
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