Diagnostic test kit and specific binding assay using...

Details Diagnostic test kit and specific binding assay using... Diagnostic test kit and specific binding assay using...

G - Physics

01

N

G01N 33/569 (2006.01) C12Q 1/28 (2006.01) G01N 33/58 (2006.01) G01N 33/74 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2077015

DIAGNOSTIC TEST KIT AND SPECIFIC BINDING ASSAY USING MODULATOR OF SIGNAL RESULTING FROM PEROXIDASE LABEL Abstract of the Disclosure The signal generated in a specific binding assay wherein a peroxidase label is used to detect the resulting specific binding complex can be modulated by contacting the signal forming reagents with a buffered solution of a hydroxamic acid or acyl hydrazine having the structure R-CO-NH-R' or an equivalent salt thereof, wherein R is aryl of 6 to 10 carbon atoms in the aromatic nucleus, alkyl of 1 to 7 carbon atoms, cycloalkyl of 5 to 10 carbon atoms in the ring or aromatic heterocyclyl of 5 to 10 atoms in the ring, at least one of which is an oxygen, nitrogen or sulfur atom, and R' is hydroxy or amino. This solution can be provided in a diagnostic test kit for use in various methods to detect a specific binding ligand. The result is improved signal stability and lowered background.

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