Diagnostics based on tetrazolium compounds

Details Diagnostics based on tetrazolium compounds Diagnostics based on tetrazolium compounds

C - Chemistry, Metallurgy

12

Q

C12Q 1/54 (2006.01) C12M 1/34 (2006.01) C12M 1/40 (2006.01) C12Q 1/32 (2006.01) G01N 33/52 (2006.01) G01N 33/64 (2006.01)

Patent

CA 2270334

A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises dehydrogenase enzyme that has specificity for the analyte, NAD, an NAD derivative, pyrrolo-quinoline quinone (PQQ), or a PQQ derivative, a tetrazolium dye precursor, a diaphorase enzyme or an analog thereof, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring ketone bodies, such as beta-hydroxybutyrate.

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