Diamine oxidase determination

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/26 (2006.01) G01N 33/84 (2006.01)

Patent

CA 2629061

Disclosed is a method for determining histaminase (DAO; EC 1.4.3.6) activity in a sample. Said method comprises the following steps: an aqueous solution is supplied which contains a predefined amount of a diamine; the aqueous solution is mixed and incubated with the sample for a given period of time in conditions in which the diamine can be reacted with a DAO possibly present in the sample; the diamine is derivatized; the amount of derivatized diamine is determined; the predefined amount of diamine is compared to the amount of derivatized diamine; and the activity of histaminase possibly present is determined.

Procédé de détermination de l'activité de diaminooxydase (DAO; EC 1.4.3.6) dans un échantillon. Ledit procédé consiste à préparer une solution aqueuse contenant une quantité prédéfinie d'une diamine, à mélanger et à faire incuber la solution aqueuse avec l'échantillon pour un laps de temps prédéterminé dans des conditions dans lesquelles la diamine peut être convertie par une DAO éventuellement présente dans l'échantillon, à dérivatiser la diamine, à déterminer la quantité de diamine dérivatisée et à comparer la quantité prédéfinie de diamine à la quantité de diamine dérivatisée, puis à déterminer l'activité de la diaminooxydase éventuellement présente.

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