Dibenzodihydropyridinecarboxylic esters and their use in...

C - Chemistry – Metallurgy – 07 – D

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C07D 219/04 (2006.01) C07D 221/12 (2006.01) C07D 221/18 (2006.01) C07D 401/00 (2006.01) C07F 9/576 (2006.01) C07F 9/64 (2006.01) C12Q 1/28 (2006.01)

Patent

CA 2261032

New dibenzodihydropyridinecarboxylic esters are provided which comply with formula (1) or (2), wherein: R1 is substituted alkyl or cyano; R2 and R3 are independently hydrogen, alkyl, substituted alkyl, aryl or substituted aryl; R4 is hydrogen, alkyl, substituted alkyl, aryl or substituted aryl; R5 and R6 are indenpendently alkyl, alkoxy or another substituent which is relatively resistant to oxidation; m and n are integers from 0 to 4; wherein, if m or n is at least 2, two groups R5 or R6 may be linked together; and Z is oxygen or sulphur. The new compounds are useful as chemiluminogenic reagents for detecting the presence of peroxidase activity at extremely low levels (10-20 moles).

L'invention concerne de nouveaux esters dibenzodihydropyridinecarboxyliques conformes aux formules (1) et (2) ci-après: dans lesquels R?1¿ est un alkyle ou un cyano substitué; R?2¿ et R?3¿ sont, indépendamment l'un de l'autre, un hydrogène, un alkyle, un alkyle substitué, un aryle ou un aryle substitué; R?4¿ est un hydrogène, un alkyle, un alkyle substitué, un aryle ou un aryle substitué; R?5¿ et R?6¿ sont, indépendamment l'un de l'autre, un alkyle, un alkoxy ou un autre substituant relativement résistant à l'oxydation; m et n sont des entiers de 0 à 4; où, si m ou n est au moins égal à 2, deux groupes R?5¿ et R?6¿ peuvent être liés ensemble; et Z est de l'oxygène ou du soufre. Les nouveaux composés sont utiles en tant que réactifs chimiluminogéniques afin de détecter la présence d'une activité de peroxydase à des taux extrêmement bas (10?-20¿ moles).

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