C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/80 (2006.01) C07K 14/445 (2006.01) C12N 1/15 (2006.01) C12N 9/10 (2006.01) C12N 15/30 (2006.01) C12N 15/52 (2006.01)
Patent
CA 2087339
The invention relates to recombinant DNA molecules comprising a Dictyostelium discoideum homologous promoter re- gion, a heterologous DNA sequence with a Dictyostelium discoideum homologous peptide sequence capable of functioning as a leader peptide sequence positioned upstream thereof and in proper reading frame therewith, and a Dictyostelium discoideum homologous termination region, the heterologous DNA sequence encoding the desired functional polypeptide or intermediate thereof, said DNA sequence being positioned downstream from the promoter region and said termination region being posi- tioned downstream from the DNA sequence. The invention also provides recombinant dictyostelid hosts as well as methods for preparing recombinant DNA molecules of the invention and for expressing recombinant polypeptides encoded by said recombi- nant DNA molecules dictyostelid hosts. The invention in particular relates to the expression of the CS protein of Plasmodium falciparum.
Fasel Nicolas J.
Reymond Christophe D.
Fetherstonhaugh & Co.
Rmf Dictagene S.a.
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