Dideoxy dye terminators

Details Dideoxy dye terminators Dideoxy dye terminators

C - Chemistry, Metallurgy

07

H

C07H 19/04 (2006.01) C07H 21/04 (2006.01) C09B 23/01 (2006.01) C09B 23/08 (2006.01) C09B 62/002 (2006.01) C09B 62/343 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2319777

A kit for DNA sequencing comprising a first, second, third and fourth dye terminator molecules, each of the dye terminator molecules comprising a dye molecule, a linker of at least 10 atoms in length and either ddATP, ddCTP, ddGTP or ddTTP as a mono or tri-phosphate and a thermostable DNA polymerase.

L'invention concerne un kit de séquençage de l'ADN comprenant une première, une deuxième, une troisième, et une quatrième molécules de terminaison de colorant, chacune de ces molécules de terminaison de colorant renfermant une molécule de colorant, un segment de liaison long d'au moins 10 atomes et ddATP, ddCTP, ddGTP, ou ddTTP, ce segment de liaison se présentant par ailleurs sous la forme d'un monophosphate ou d'un triphosphate, et une ADN polymérase thermostable.

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