Differential agars medium for separating streptococcus...

Details Differential agars medium for separating streptococcus... Differential agars medium for separating streptococcus...

C - Chemistry, Metallurgy

12

N

195/40

C12N 1/20 (2006.01)

Patent

CA 1322733

ABSTRACT OF THE DISCLOSURE The characteristic ability of Streptococcus faecalis and inability of Streptococcus lactis to produce the butyrate esterase formed the basis for the development of a differential agar medium to separate these species in pure and mixed cultures. The differential agar medium contained 0.5% bacto-peptone, 0.3% bacto-yeast extract, 1.0% 2-naphthyl butyrate or 1.0% indoxyl butyrate, 1.5% agar and the remaining 96.7% water (pH 6.5). S. faecalis colonies grown on naphthyl butyrate agar (24 h; 30°C) produced an intense violet color on immediately after the addition of detector reagents, while S. lactis did not. A solid medium with indoxyl butyrate as indicator was found suitable for both qualitative and quantitative differentiation by direct observation of indigo blue formation around the colonies of S. faecalis. This is a first rapid new medium developed for separating S. faecalis and S. lactis colonies without inhibition.

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