Differential binding affinities and dissociation assays...

Details Differential binding affinities and dissociation assays... Differential binding affinities and dissociation assays...

G - Physics

01

N

G01N 33/53 (2006.01) G01N 33/58 (2006.01) G01N 33/72 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2114440

A method for assaying for the presence of analyze in a sample based on differential binding affinity involves detecting dissociation of a complex of receptor and ligand in the presence of analyte, The receptor binds the analyte with high affinity and with the ligand with low affinity. The receptor-ligand complex may be formed in situ or may be preformed. In the presence of free analyte, the receptor releases from the receptor-ligand complex and binds free analyte. Release of the receptor-ligand complex is detectable. A kit for performing release assays to detect the presence of analyte is also provided.

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