Differential expression of molecules associated with acute...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2572795

Methods are provided for evaluating a stroke, for example for determining whether a subject has had an ischemic stroke, determining the severity or likely neurological recovery of a subject who has had an ischemic stroke, and determining a treatment regimen for a subject who has had an ischemic stroke, as are arrays and kits that can be used to practice the methods. In particular examples, the method includes screening for expression in ischemic stroke related genes (or proteins), such as white blood cell activation and differentiation genes (or proteins), genes (or proteins) related to hypoxia, genes (or proteins) involved in vascular repair, and genes (or proteins) related to a specific peripheral blood mononuclear cell (PBMC) response to the altered cerebral microenvironment.

L'invention concerne des méthodes d'évaluation d'un accident vasculaire cérébral permettant, par exemple, de déterminer si un patient a eu un accident ischémique cérébral, de déterminer la sévérité ou la récupération neurologique probable d'un patient ayant souffert d'un accident ischémique cérébral, et de déterminer un régime de traitement pour le patient ayant souffert d'un accident ischémique cérébral, ainsi que des jeux d'échantillons et des kits pouvant être utilisés dans la mise en oeuvre desdites méthodes. Dans des exemples particuliers, la méthode consiste à effectuer un criblage pour l'expression des gènes (ou protéines) associés à un accident ischémique cérébral, par exemple les gènes (ou protéines) d'activation et de différenciation des leucocytes, les gènes (ou protéines) associés à l'hypoxie, les gènes (ou protéines) associés à la réparation vasculaire, et les gènes (ou protéines) associés à une réponse spécifique des cellules mononucléaires sanguines périphériques à un micro-environnement cérébral altéré.

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