Differential expression of organellar gene products

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) C07K 14/47 (2006.01) C12N 5/10 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2363496

Compositions and methods are provided for identifying factors, including organellar factors, that are differentially expressed when cells in different states, such as metabolic, respiratory, disease or apoptotic states, are compared. In preferred embodiments the invention relates to mitochondria DNA depleted (.rho.?0~) and cytoplasmic hybrid (cybrid) cells, such as mitochondrial cybrid cells. Use of the invention to identify species specific expression of organellar factors such as organelle associated macromolecules is contemplated. Also disclosed are examples of organellar factors that are differentially expressed in organelle associated disease, including a variety of human genes that are differentially expressed in Alzheimer's disease.

L'invention a trait à des compositions et des méthodes d'identification de facteurs, y compris des facteurs d'organites, qui sont exprimés de manière différentielle, lorsque des cellules dans des états différents, tels que des états métaboliques, respiratoires, malades ou apoptiques, sont comparées. Selon des modes de réalisation préférés, l'invention concerne des cellules ( rho <0>) dépourvues d'ADN de mitonchondrie et des cellules hybrides cytoplasmiques (cybrides), telles que les cellules cybrides mitochondriales. On envisage l'utilisation de cette invention pour identifier l'expression spécifique d'espèces de facteurs d'organites tels que l'organite associé à des macromolécules. L'invention concerne également des exemples de facteurs d'organites qui sont exprimés de manière différentielle dans une maladie associée à l'organite, ainsi qu'une variété de gènes humains qui sont exprimés de manière différentielle dans la maladie d'Alzheimer.

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