Differential genetic display technique and vector

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2342903

This invention discloses a method of determining differ- ential display of gene expression comprising comparisons of mono-length cRNA libraries. These libraries are probe hy- bridized to accessible ordered arrays to determine differential hybridization display sites between mono-length segment li- braries and to locate genes of expression differential. Also disclosed is a mono-length library corresponding to or com- plementary with expressed mRNA sequences having cRNA elements 23 nucleotides in length of the formula 5'-gcugga- gaucggnnnnnnnnnnn-3', and wherein n represents one of 11 ribonucleotides in sequence corresponding to a complementary 11 nucleotide sequence of the mRNA.

L'invention porte sur un procédé permettant d'obtenir une représentation différentielle de l'expression de gènes par comparaison de bibliothèques d'ARNc mono-longueurs. Ces bibliothèques sont hybridées par des sondes en réseaux ordonnés accessibles permettant de reconnaître les sites présentant des hybridations parmi les bibliothèques de segments mono-longueurs, et de localiser les gènes présentant un différentiel d'expression. L'invention porte également sur une bibliothèque de mono-longueurs correspondant à des, ou complémentaires de, séquences d'ARNm exprimé, comportant les éléments d'ARNc de 23 nucléotides de long et de formule 5'-gcuggagaucggnnnnnnnnnnn-3', dans laquelle n représente l'un des 11 ribonucléotides de la séquence correspondant à la séquence complémentaire de 11 nucléotides de l'ARNm.

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