Differential immunoassay

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) C07H 21/00 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/549 (2006.01) G01N 33/566 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2420327

The invention provides assay methods and kits that in general measure the level of a first analyte in a sample reduced by the level of a second analyte present in the same sample. In one embodiment, where levels of a first analyte from a first source is desirably determined and first analyte in the sample released from a second source is accompanied by proportional co-release of a second analyte, the assay identifies the level of first analyte released only from the first source. For analytes within bodily fluids, the assay can differentiate between elevated levels of analyte specific to the particular physiological or pathological state and elevated levels not specific to the particular state, providing single tests with diagnostic utility.

L'invention porte sur des kits et sur des méthodes de dosage qui permettent de mesurer en général le taux d'un premier analyte dans un échantillon réduit par le taux d'un second analyte présent dans ce même échantillon. Selon une réalisation, on détermine le taux d'un premier analyte à partir d'une première source et on libère le premier analyte de l'échantillon d'une seconde source par colibération proportionnelle d'un second analyte. Le dosage identifie le taux du premier analyte libéré uniquement de la première source. Pour des analytes se trouvant dans des fluides biologiques, le dosage peut différencier les taux élevés d'un analyte spécifique à un état physiologique ou pathologique donné des taux élevés d'un analyte spécifique à l'état donné, ce qui permet d'obtenir des tests uniques utiles dans les diagnostics.

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