Differential separation assay methods and test kits

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G01N 33/577 (2006.01) C07K 14/765 (2006.01) G01N 27/447 (2006.01) G01N 33/558 (2006.01) G01N 33/561 (2006.01) G01N 33/566 (2006.01) G01N 33/571 (2006.01) G01N 33/573 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2141802

2141802 9403631 PCTABS00030 Methods, test kits, and an apparatus for detecting analytes among closely related substances by reacting the analyte in a test sample with a labeled binding agent which specifically binds to the analyte to form a complex are disclosed. The complex and labeled binding agent are isolated on a separation medium and identified by differential rate of migration. A second labeled marker which migrates independently of the labeled binding agent and complex, preferably at or near the solvent front provides a quality control check for the assay.

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