Differentiated cells suitable for human therapy

Details Differentiated cells suitable for human therapy Differentiated cells suitable for human therapy

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) C12N 5/071 (2010.01) C12N 5/0735 (2010.01) A61K 31/522 (2006.01) A61K 35/12 (2006.01) A61K 38/45 (2006.01) A61K 48/00 (2006.01) C12N 15/09 (2006.01) C12N 9/12 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2434760

This invention provides a system for producing differentiated cells from a stem cell population for use wherever a relatively homogenous cell population is desirable. The cells contain an effector gene under control of a transcriptional control element (such as the TERT promoter) that causes the gene to be expressed in relatively undifferentiated cells in the population. Expression of the effector gene results in depletion of undifferentiated cells, or expression of a marker that can be used to remove them later. Suitable effector sequences encode a toxin, a protein that induces apoptosis, a cell-surface antigen, or an enzyme (such as thymidine kinase) that converts a prodrug into a substance that is lethal to the cell. The differentiated cell populations produced according to this disclosure are suitable for use in tissue regeneration, and non-therapeutic applications such as drug screening.

L'invention concerne un système servant à produire des cellules différenciées à partir d'une population de cellules souches et conçu pour être mis en application lorsqu'on désire obtenir une population cellulaire relativement homogène. Ces cellules contiennent un gène effecteur contrôlé par un élément de transcription (tel que le promoteur TERT) provoquant l'expression du gène dans des cellules relativement indifférenciées de la population. L'expression de ce gène effecteur provoque l'appauvrissement de cellules indifférenciées ou l'expression d'un marqueur pouvant être utilisé afin de supprimer ces cellules ultérieurement. Des séquences effectrices appropriées codent une toxine, une protéine induisant l'apoptose, un antigène de surface cellulaire ou un enzyme (tel que thymidine kinase) convertissant un promédicament en une substance mortelle pour la cellule. Ces populations de cellules différenciées préparées selon l'invention sont utilisées avantageusement pour la régénération tissulaire, ainsi que dans des mises en application non thérapeutiques, telles que le criblage de médicaments.

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