Differentiation of monocytes into functional dendritic cells

A - Human Necessities – 61 – K

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A61K 48/00 (2006.01) C12N 5/078 (2010.01) C12N 5/0784 (2010.01) A61K 35/14 (2006.01) A61K 39/00 (2006.01) A61K 41/00 (2006.01)

Patent

CA 2368855

A method for inducing differentiation of monocytes contained in an extracorporeal quantity of a subject's blood into functional dendritic antigen presenting cells is provided. The monocytes are first treated by exposure to physical perturbation, irradiation in the presence of a photoactivatable agent capable of forming photoadducts with cellular DNA components, and/or treatment with a DNA binding agent. The treated monocytes are then incubated for a period of time sufficient to maximize the number of functional dendritic cells in the treated cell population. Functional dendritic cells generated from induced monocytes are incubated together with disease effector agents to enhance the presentation of at least one disease-causing antigen expressed by the disease effector agents. Compositions including dendritic cells derived from induced monocytes and compositions including such dendritic cells incubated with disease effector agents are also provided.

L'invention concerne un procédé induisant la différentiation de monocytes contenus dans un volume extracorporel de sang prélevé chez un patient et transformés en cellules dendritiques fonctionnelles présentant un antigène. Les monocytes sont d'abord exposés à une perturbation physique, à un rayonnement en présence d'un agent photoactivable capable de former des composés d'addition avec des composants cellulaires de l'ADN et/ou traités avec un agent de liaison à l'ADN. Les monocytes traités sont ensuite incubés pendant une période de temps suffisante pour augmenter au maximum le nombre de cellules dendritiques fonctionnelles dans la population de cellules traitées. Les cellules dendritiques fonctionnelles issues des monocytes induits sont incubées avec des agents effecteurs de maladie afin d'augmenter la présentation d'au moins un antigène à l'origine de la maladie exprimé par les agents effecteurs de maladie. L'invention concerne également des compositions renfermant des cellules dendritiques issues des monocytes induits et des compositions renfermant des cellules dendritiques incubées avec des agents effecteurs de maladie.

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