Differentiation of pluripotent cells

C - Chemistry – Metallurgy – 12 – N

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C12N 5/0781 (2010.01) C12N 5/071 (2010.01)

Patent

CA 2753679

Provided herein are methods for the in vitro maintenance, expansion, culture, and/or differentiation of pluripotent cells, such as human embryonic stem cells (hESC) or induced pluripotent cells (iPSC), into hematopoietic precursor cells or endothelial cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the pluripotent cells into hematopoietic precursor cells or endothelial cells. The resulting hematopoietic precursor cells may be further differentiated into various myeloid or lymphoid lineages.

La présente invention concerne des méthodes permettant la conservation, l'expansion, la culture et/ou la différenciation in vitro de cellules pluripotentes, telles que des cellules souches embryonnaires humaines (hESC) ou des cellules pluripotentes induites (iPSC), en cellules précurseurs hématopoïétiques ou en cellules endothéliales. Les cellules pluripotentes peuvent être conservées et différentiées dans des conditions définies; ainsi, l'utilisation de cellules nourricières de souris ou de sérum n'est pas nécessaire dans certains modes de réalisation pour la différenciation des cellules pluripotentes en cellules précurseurs hématopoïétiques ou en cellules endothéliales. Les cellules précurseurs hématopoïétiques obtenues peuvent être davantage différenciées en diverses lignées myéloïdes ou lymphoïdes.

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